Case study assessment and analysis of physiological biomarkers in an equine post inoculation with Bothrops sp. venom.

Abstract

This document analyzes the inoculation into the Bothrops venom in an equine of Creole breed taking into account the properties and characteristics from this species, such as robustness, resistance and production immunoglobulins capacity, also some local physical changes were likewise observed in the place of the Inoculation and nearly behavioral changes that this equine presented during the procedure, in addition, the comparison of biochemical tests was carried out in order to keep track during the process. The equine was inoculated using the method described by De Roodt, the inoculation was performed on days 1, 15, 30, 45, 55 and 65, with doses of venom established according to the LD50 of the poison in the table, in the neck at subcutaneous level at different points on a mid-lateral line parallel to the mane, in the craniocaudal direction. The local lesions caused were produced by Freund’s adjuvant. In the erythrogram of the first and last doesn´t report significant differences stablishing normal biological range except for the width from the erythrocyte distribution that indicated anisocytosis. In the leukogram, the first and last shots were considered, issues in leukocytosis through neutrophilia without deviation to the left. Among the biochemical indicators, a decrease in ALT was found from the second dose to the last and the AST. At the end in all cases was found within the reference values. With the protocol carried out, no several effects were generated that put the health or integrity of the horse at risk, however, some insignificant behavioral and biochemicals changes were perceptible.